Understanding microsecond dynamics of protein machines with single-molecule fluorescence

Seminar
QUEST Center event
No
Speaker
Gilad Haran, Weizmann Institute of Science
Date
24/12/2018 - 12:30Add to Calendar 2018-12-24 12:30:00 2018-12-24 12:30:00 Understanding microsecond dynamics of protein machines with single-molecule fluorescence Protein machines carry out specific tasks in the cell by alternating chemical steps with conformational/structural transitions. Single-molecule fluorescence spectroscopy is a powerful tool for exposing large-scale function-related motions. We recently developed a sophisticated maximum likelihood algorithm for the analysis of single-molecule experiments, which can track conformational dynamics even on the microsecond time scale. In the lecture, I will show how this novel analysis helped us understanding the dynamics of two machines, an abundant enzyme and a protein that rescues other proteins from aggregation.  Physics 301 המחלקה לפיזיקה physics.dept@mail.biu.ac.il Asia/Jerusalem public
Place
Physics 301
Abstract

Protein machines carry out specific tasks in the cell by alternating chemical steps with conformational/structural transitions. Single-molecule fluorescence spectroscopy is a powerful tool for exposing large-scale function-related motions. We recently developed a sophisticated maximum likelihood algorithm for the analysis of single-molecule experiments, which can track conformational dynamics even on the microsecond time scale. In the lecture, I will show how this novel analysis helped us understanding the dynamics of two machines, an abundant enzyme and a protein that rescues other proteins from aggregation. 

תאריך עדכון אחרון : 11/12/2018