Single-Molecule Studies of Proteins DNA and their Interactions

Single-molecule methods are providing new and powerful tools for exploring proteins folding, DNA conformations and protein-DNA interactions. These processes are important in cellular activities, and their understanding requires to combine substantial interesting physical and biological considerations.

I will present a method we developed for measuring and characterizing individual protein-folding pathways and DNA-protein interactions based on tethered particle motion (TPM). Combining the TPM data with force and AFM measurements provides important insight on DNA-protein interactions and protein folding.