MINFLUX nanoscopy and related matters

Seminar

QUEST Center event

Speaker

Prof. Stefan W. Hell, Max Planck Institute for Biophysical Chemistry, Göttingen & Max Planck Institute for Medical Research, Heidelberg

Date

22/03/2021 - 20:00 - 18:00Add to Calendar 2021-03-22 18:00:00 2021-03-22 20:00:00 MINFLUX nanoscopy and related matters Zoom Details: Meeting ID: 951 7512 2285 Password: 071333 Add to Google Calendar I will show how an in-depth description of the basic principles of diffraction-unlimited fluorescence microscopy (nanoscopy) [1-3] has spawned a new powerful superresolution concept, namely MINFLUX nanoscopy [4]. MINFLUX utilizes a local excitation intensity minimum (of a doughnut or a standing wave) that is targeted like a probe in order to localize the fluorescent molecule to be registered. In combination with single-molecule switching for sequential registration, MINFLUX [4-6] has obtained the ultimate (super)resolution: the size of a molecule. MINFLUX nanoscopy, providing 1–3 nanometer resolution in fixed and living cells, is presently being established for routine fluorescence imaging at the highest, molecular-size resolution levels. Relying on fewer detected photons than popular camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells. [1] Hell, S.W., Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission- depletion fluorescence microscopy. Opt. Lett. 19, 780-782 (1994). [2] Hell, S.W. Far-Field Optical Nanoscopy. Science 316, 1153-1158 (2007). [3] Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24-32 (2009). [4] Balzarotti, F., Eilers, Y., Gwosch, K. C., Gynnå, A. H., Westphal, V., Stefani, F. D., Elf, J., Hell, S.W. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606-612 (2017). [5] Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., Hell, S. W. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. PNAS 115, 6117-6122 (2018). [6] Gwosch, K. C., Pape, J. K., Balzarotti, F., Hoess, P., Ellenberg, J., Ries, J., Hell, S. W. MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells. (bioRxiv, doi: https://doi.org/10.1101/734251) Zoom Department of Physics physics.dept@mail.biu.ac.il Asia/Jerusalem public

Place

Zoom

Abstract

Zoom Details:

Meeting ID: 951 7512 2285

Password: 071333

Add to Google Calendar

I will show how an in-depth description of the basic principles of diffraction-unlimited fluorescence microscopy (nanoscopy) [1-3] has spawned a new powerful superresolution concept, namely MINFLUX nanoscopy [4]. MINFLUX utilizes a local excitation intensity minimum (of a doughnut or a standing wave) that is targeted like a probe in order to localize the fluorescent molecule to be registered. In combination with single-molecule switching for sequential registration, MINFLUX [4-6] has obtained the ultimate (super)resolution: the size of a molecule. MINFLUX nanoscopy, providing 1–3 nanometer resolution in fixed and living cells, is presently being established for routine fluorescence imaging at the highest, molecular-size resolution levels. Relying on fewer detected photons than popular camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.

[1] Hell, S.W., Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission- depletion fluorescence microscopy. Opt. Lett. 19, 780-782 (1994).

[2] Hell, S.W. Far-Field Optical Nanoscopy. Science 316, 1153-1158 (2007).

[3] Hell, S.W. Microscopy and its focal switch. Nat. Methods 6, 24-32 (2009).

[4] Balzarotti, F., Eilers, Y., Gwosch, K. C., Gynnå, A. H., Westphal, V., Stefani, F. D., Elf, J., Hell, S.W. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science 355, 606-612 (2017).

[5] Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., Hell, S. W. MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution. PNAS 115, 6117-6122 (2018).

[6] Gwosch, K. C., Pape, J. K., Balzarotti, F., Hoess, P., Ellenberg, J., Ries, J., Hell, S. W. MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution in (living) cells. (bioRxiv, doi: https://doi.org/10.1101/734251)

Attached file

22.03_Stefan Hell[65015].pdf

Last Updated Date : 05/12/2022